Developmental competence of porcine genome-edited zygotes
نویسندگان
چکیده
منابع مشابه
Live pigs produced from genome edited zygotes.
Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly br...
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The Aggregation of Four Reconstructed Zygotes is the Limit to Improve the Developmental Competence of Cloned Equine Embryos
Embryo aggregation has been demonstrated to improve cloning efficiency in mammals. However, since no more than three embryos have been used for aggregation, the effect of using a larger number of cloned zygotes is unknown. Therefore, the goal of the present study was to determine whether increased numbers of cloned aggregated zygotes results in improved in vitro and in vivo embryo development i...
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ژورنال
عنوان ژورنال: Molecular Reproduction and Development
سال: 2017
ISSN: 1040-452X
DOI: 10.1002/mrd.22829